Chemical characterization and biologic properties of lipopolysaccharide from Bacteroides gingivalis strains W50, W83, and ATCC 33277
- 1 August 1989
- journal article
- research article
- Published by Wiley in Oral Microbiology and Immunology
- Vol. 4 (4), 183-192
- https://doi.org/10.1111/j.1399-302x.1989.tb00250.x
Abstract
The chemistry and selected biological activity of lipopolysaccharide (LPS) from Bacteroides gingivalis strains W50, E83, and ATCC 33277 were compared, as well as the role of this molecule as a mediator of selected inflammatory responses. Chemically, the LPSs consisted of 47-58% Lipid A, 5-10% carbohydrate, 0.05% 3-deoxy 2-octulosonic acid, 0.3% heptose, 3.8-5.2% hexosamine,and 2% phosphate. Rhamnose represented the dominant sugar (26-36%), with lesser amounts of glucose (18-34%), galactose (18-25%), mannose (9-12%), glucosamine (7-11%), and galactosamine (2-5%). The major fatty acids were: 13-methyltetradecanoate (42-45%), 3-OH-heptadecanoate (21-23%), hexadecanoate (16-19%),and 12-methyl-tetradecanoate (6-8%). SDS-PAGE and sodium deoxycholate-PAGE revealed the LPS to be a smooth chemotype. Differences in migration patterns between the virulent and avirulent strain LPSs also occurred. C3H/HeN macrophages (M.phi.) exposed to 1 .mu.g/ml of LPS released 3.2-4.2 ng of prostaglandin E (PGE)/ml of supernatant, representing 236-278% of control. Interleukin-1 (IL-1) activity in C3H/HeN and C3H/HeJ M.phi. exposed to 50 .mu.g of LPS/ml was 382-724% and 270-300% of control, respectively; similar M.phi. exposed to 10 .mu.g of LPS/ml released 1.6-2.0 ng and 0.3-0.5 ng of tumor necrosis factor (TNF)/ml of supernatant, respectively. Maximum TNF release in C3H/HeN M.phi. occurred in response to 50 .mu.g of LPS/ml, and was sustained for up to 96 hours. These results suggest that LPS from the B. gingivalis strains stimulate cytokine production from M.phi. which, in turn, may play a role in orchestrating the inflammatory response for the development of periodontal diseases.Keywords
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