Actions of aldosterone on polyadenylated ribonucleic acid and Na+ ion transport in the toad bladder

Abstract

Polyadenylated RNA [poly(A)(+)-RNA] was isolated from the cytoplasm of the epithelium of the urinary bladder of the toad (Bufo marinus] by oligo-(deoxythymidylate)cellulose chromatography. Aldosterone increased the incorporation of [3H]uridine and of [3H]uridine and [3H]adenosine (given in combination) into 7S, 12S and 18S poly(A)(+)-RNA during the first 30 min of the action of the hormone, as defined by either a pulse or a pulse-chase sequence. The quantity of cytoplasmic poly(A)(+)-RNA that hybridized to [3H]poly(uridine) was also increased by aldosterone. These results are consistent with the inference of induction of mRNA synthesis. This effect was most marked during the first 30 min of the action of the hormone.