Direct spectroscopic observation of inner and outer hydrocarbon chains of lipid bilayer vesicles.
- 1 August 1976
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 73 (8), 2716-2719
- https://doi.org/10.1073/pnas.73.8.2716
Abstract
Distearoylphosphatidylcholines labeled with gem-difluoro groups at selected positions of the fatty acid chains were prepared. These lipids were incorporated into sonicated vesicles and were examined by F NMR spectroscopy. All spectra are characterized by 2 well-separated F resonances. Paramagnetic ions added to the exterior solution of vesicles of fluorinated lipid broaden the upfield resonances, ions trapped in the vesicle interior broaden the downfield resonances. The F chemical shift of these compounds is sensitive to differences between the inner and outer monolayers of sonicated vesicles.This publication has 10 references indexed in Scilit:
- Molecular motion and order in single-bilayer vesicles with multilamellar dispersions of egg lecithin and lecithin-cholesterol mixtures. A deuterium nuclear magnetic resonance study of specifically labeled lipidsBiochemistry, 1976
- Differential scanning calorimetry and 31P NMR studies on sonicated and unsonicated phosphatidylcholine liposomesBiochimica et Biophysica Acta (BBA) - Biomembranes, 1975
- A novel synthesis of spin label derivatives of phosphatidylcholineAnalytical Biochemistry, 1975
- Dynamic structure of fatty acyl chains in a phospholipid bilayer measured by deuterium magnetic resonanceBiochemistry, 1974
- Differential Interaction of Cholesterol with Phosphatidylcholine on the Inner and Outer Surfaces of Lipid Bilayer VesiclesProceedings of the National Academy of Sciences, 1974
- Effect of sonication on the structure of lecithin bilayersBiochemistry, 1972
- Synthesis of lecithins by acylation of O-(sn-glycero-3-phosphoryl) choline with fatty acid anhydridesBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1969
- A spin-labeled lipid for probing biological membranesChemistry and Physics of Lipids, 1969
- Phospholipase A. I. Isolation and characterization of two enzymes from Crotalus adamanteus venomBiochemistry, 1969