Studies on the α-subunit of bovine brain S-100 protein
- 15 March 1984
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 218 (3), 691-696
- https://doi.org/10.1042/bj2180691
Abstract
A method is described for the rapid purification of both S-100 protein and calmodulin from crude bovine brain extracts by the use of a fluphenazine-Sepharose affinity column eluted stepwise with decreasing concentrations of free Ca2+. Protein containing only alpha-subunit was purified from preparations of S-100 protein by anion-exchange chromatography. This protein co-migrated with the alpha-subunit of S-100 protein on sodium dodecyl sulphate/urea/polyacrylamide-gel electrophoresis and had an amino acid composition identical with that previously reported for this subunit. The results of u.v.-absorption and fluorescence-emission spectroscopy indicate that the tryptophan residue of the purified alpha-subunit of S-100 protein undergoes a Ca2+-induced change in environment. Measurements of changes in tryptophan fluorescence with increasing Ca2+ concentrations suggest an apparent dissociation constant of the alpha-subunit for Ca2+ of 7 × 10(-5)M in the absence of K+. In the presence of 90mM-K+ this value is increased to 3.4 × 10(-4)M.This publication has 17 references indexed in Scilit:
- The Amino Acid Sequence of the Tryptophan‐Containing Subunit (α‐Subunit) of Bovine Brain S‐100 ProteinJournal of Neurochemistry, 1981
- Structural Relation of Two S-100 Proteins in Bovine Brain; Subunit Composition of S-100 a ProteinEuropean Journal of Biochemistry, 1981
- Heterogeneity of S-100 protein: Comparison of bovine and guinea-pig S-100 proteinsInternational Journal of Biochemistry, 1981
- Purification of plant calmodulin by fluphenazine-Sepharose affinity chromatographyBiochemical and Biophysical Research Communications, 1979
- HETEROGENEITY OF S‐100 PROTEINS OF BRAIN: SUBUNIT COMPOSITIONSJournal of Neurochemistry, 1979
- Magnesium and calcium binding to parvalbumins: evidence for differences between parvalbumins and an explanation of their relaxing functionBiochemistry, 1979
- CALCULATOR PROGRAMS FOR COMPUTING THE COMPOSITION OF THE SOLUTIONS CONTAINING MULTIPLE METALS AND LIGANDS USED FOR EXPERIMENTS IN SKINNED MUSCLE-CELLS1979
- The amino acid sequence and the subunit structure of bovine brain S‐100 protein (PAP I‐b)Journal of Neurochemistry, 1978
- Reinvestigation of extremely acidic proteins in bovine brainBiochimica et Biophysica Acta (BBA) - Protein Structure, 1977
- PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENTJournal of Biological Chemistry, 1951