Effects of Dietary Fat Level and Dimethylhyd,razine on Fecal Acid and Neutral Sterol Excretion and Colon Carcinogenesis in Rats 2

Abstract

We studied the effect of dietary fat on the induction of colon tumors by 1,2-dimethylhydrazine (DMH) and that of dietary fat and DMH on the excretion of acid and neutral sterols in rat feces. High fat (20%) intake was associated with an increased fecal acid and neutral sterol excretion. Fecal acid sterols were more extensively degraded in rats fed a high-fat diet than on those fed low-fat (0.5%) or normal-fat (5%) diets. DMH-treated animals excreted high levels of acid and neutral sterols and more microbially modified acid steroids than did controls. Animals fed a high-fat diet were more susceptible to colon tumor induction by DMH compared to rats fed a low-, normal-fat, or Purina Lab Chow diet.