Rat 7S immunoglobulins: characterization of gamma-2- and gamma-1-anti-haptens antibodies.

Abstract

Two antigenically distinct populations of rat antibodies to the 2,4-dinitrophenyl group (DNP) have been isolated by chromatography on DEAE-cellulose and named fraction 1 and fraction 3. Both fraction 1 and fraction 3 had sedimentation coefficients of 6.9S, similar hexosamine contents (1.04 and 0.89 per cent) and electrophoretic mobilities similar to the γ2- and γ1-immunoglobulins of guinea-pigs and mice. Fraction 1 consisted of two distinct antibody populations with very similar mobilities.