Respiratory metabolism in rat tissues after autolysis in situ
- 1 August 1965
- journal article
- research article
- Published by American Physiological Society in American Journal of Physiology-Legacy Content
- Vol. 209 (2), 438-442
- https://doi.org/10.1152/ajplegacy.1965.209.2.438
Abstract
Rat tissues were subjected to autolysis in situ by incubation of the decapitated carcass at 37 C. After variable periods, samples of liver, kidney, and brain were either frozen in liquid propane (–190 C) for measurement of ATP or homogenized in sucrose-EDTA medium for polarographic measurements. The rate of disappearance of ATP was in the order brain > liver > kidney, and the "stable" ATP concentration after 60-min autolysis was greatest in kidney and least in brain. After 90-min autolysis, the "basal" (nonstimulated) oxygen consumption of homogenates was either increased (liver) or unchanged (kidney, brain), and the ADP-stimulated oxygen consumption was depressed in all three tissues. Respiratory control (stimulation of oxygen uptake by ADP) was abolished in brain after 60-min autolysis and in liver and kidney after 90 min when the substrates were alpha-ketoglutarate or glutamate; some control was observed when succinate was the substrate. Oxidative phosphorylation (ADP:O ratio) was not affected by 60-min autolysis. It is concluded that autolysis of 60-min duration results in depression of the rate at which ATP can be synthesized without affecting the energetic efficiency of ATP formation.Keywords
This publication has 11 references indexed in Scilit:
- AN ELECTRON MICROSCOPIC STUDY OF EARLY CYTOPLASMIC ALTERATIONS IN HEPATIC PARENCHYMAL CELLS OF MOUSE LIVER DURING NECROSIS IN VITRO (AUTOLYSIS)1962
- Functional changes in liver mitochondria following in situ anoxiaAmerican Journal of Physiology-Legacy Content, 1960
- METABOLIC AND STRUCTURAL STATES OF MITOCHONDRIA .1. REGULATION BY ADENOSINE DIPHOSPHATE1960
- Factors influencing the survival of liver cells during autolysisThe Journal of Pathology and Bacteriology, 1959
- Function of adenosine triphosphate in the activation of luciferinArchives of Biochemistry and Biophysics, 1956
- Enzyme changes during liver autolysisThe Journal of Pathology and Bacteriology, 1956
- RESPIRATORY ENZYMES IN OXIDATIVE PHOSPHORYLATION .3. THE STEADY STATE1955
- Effects of Anoxia on Respiratory Enzymes of the Rat and HamsterAmerican Journal of Physiology-Legacy Content, 1954
- Firefly luminescence in the study of energy transfer mechanisms. I. Substrate and enzyme determinationArchives of Biochemistry and Biophysics, 1952
- Mitochondrial and pH changes in the rat's kidney following interruption and restoration of the renal circulationThe Anatomical Record, 1940