Cell cycles in two cell lines of human bladder carcinoma.
- 1 January 1977
- journal article
- research article
- Published by Tohoku University Medical Press in The Tohoku Journal of Experimental Medicine
- Vol. 121 (2), 157-164
- https://doi.org/10.1620/tjem.121.157
Abstract
Cell cycle analysis was done in established cell lines of human bladder transitional carcinoma, MGH-U1 and T24 cells. The cells were cultured on round cover slips for 3 days, autoradiograms were prepared after pulse labeling with 3H-TdR [3H-thymidine] and the fractions of labeled mitoses (FLM) were calculated every 2 h through 36 h. The FLM curves were analyzed by the empirical half height level method as well as Mendelsohn-Takahashi''s asymmetry method which is a global type of FLM analysis based on Takahashi''s generalized model. The results did not show any distinct differences in cell cycle parameters between MGH-U1 and T24 cell lines. A remarkable discrepancy was observed between the cell cycle times analyzed by the asymmetry method and the half height level method. Although there is no evidence that the asymmetry method is biologically correct up to now, Mendelsohn and Takahashi tested this method repeatedly and mathematically proved that it had less error than that of the traditional boundary method.Keywords
This publication has 11 references indexed in Scilit:
- DNA synthesis in 5-bromouracil tolerant HeLa cellsExperimental Cell Research, 1967
- QUANTITATIVE ANALYSIS OF CELL PROLIFERATION AND DIFFERENTIATION IN THE CORTEX OF THE POSTNATAL MOUSE CEREBELLUMThe Journal of cell biology, 1967
- A Mathematical Model of the Mitotic Cycle and Its Application to the Interpretation of Percentage Labeled Mitoses Data2JNCI Journal of the National Cancer Institute, 1966
- Inhibition of the division cycle in confluent cultures of human fibroblasts in vitroExperimental Cell Research, 1966
- The division cycle and RNA-synthesis in diploid human cells at different passage levels in vitroExperimental Cell Research, 1966
- Measurement of DNA-synthesis time in myeloid-erythroid precursorsExperimental Cell Research, 1965
- Duration of DNA Synthesis in Ehrlich Ascites Cells as Estimated by Double-Labeling With C14- and H3-Thymidine and Autoradiography2JNCI Journal of the National Cancer Institute, 1963
- A 14C- and 3H-thymidine double labeling technique in the study of cell proliferation in Tradescantia root tipsExperimental Cell Research, 1963
- DNA synthesis in individual L-strain mouse cellsBiochimica et Biophysica Acta, 1959
- Cell population kinetics in the intestinal epithelium of the mouseExperimental Cell Research, 1959