Acute‐phase protein detection and quantification in gingival crevicular fluid by direct and indirect immunodot
- 1 February 1991
- journal article
- Published by Wiley in Journal of Clinical Periodontology
- Vol. 18 (2), 101-106
- https://doi.org/10.1111/j.1600-051x.1991.tb01697.x
Abstract
The development of an assay for markers of active periodontitis, obtained directly from gingival crevicular fluid (GCF) and simply quantified, would be of great importance to the dental practitioner. The purpose of this study was to evaluate direct and indirect immunodot techniques as to their potential in easily quantifying acute-phase proteins within periodontally diseased and healthy site GCF. Indirect immunodots (GCF eluates dotted onto nitrocellulose membrane) using monoclonal antibodies and a radioactive isotope label were used to identify and establish relative amounts of C-reactive protein (CRP) and alpha-2-macroglobulin (A2M) in 2 diseased and 2 healthy sites in 24 periodontitis patients. Periodontally diseased sites were found to contain significantly lower concentrations of A2M than healthy sites (p less than 0.001), but CRP levels did not vary significantly between healthy and diseased locations. Using a direct immunodot assay (GCF absorbed directly into nitrocellulose membrane strips), A2M levels quantified with radioactive isotopes at healthy and diseased sites could be correlated with A2M levels determined by enzyme-linked antibody-colormetric probes at those same sites. Such a direct sampling and quantification system shows promise for future "in-office" diagnostic methodology.Keywords
This publication has 19 references indexed in Scilit:
- Quantitative dot-blot enzyme immunoassay for serum amyloid A proteinJournal of Immunological Methods, 1989
- Binding of alpha-2-macroglobulin trypsin complex to human monocytes in cultureScandinavian Journal of Clinical and Laboratory Investigation, 1987
- Immunoblotting with monoclonal antibodies: Importance of the blocking solutionAnalytical Biochemistry, 1986
- Alpha 2-macroglobulin in gingival fluid: correlation with alveolar bone loss in periodontal diseaseJournal of Clinical Periodontology, 1986
- Immunoblotting and dot immunobinding — Current status and outlookJournal of Immunological Methods, 1984
- A rapid, sensitive method for detection of alkaline phosphatase-conjugated anti-antibody on Western blotsAnalytical Biochemistry, 1984
- Detection of antigens on nitrocellulose paper immunoblots with monoclonal antibodiesAnalytical Biochemistry, 1983
- Protein blotting: Principles and applicationsAnalytical Biochemistry, 1983
- Modulation of the Immune Response by Plasma Protease InhibitorsScandinavian Journal of Immunology, 1982
- Immuno-Electrophoretic Study of Tissue Fluid from Gingival PocketsActa Odontologica Scandinavica, 1960