Somatostatin Modulates Voltage-Gated K+and Ca2+Currents in Rod and Cone Photoreceptors of the Salamander Retina

Abstract

We investigated the cellular localization in the salamander retina of one of the somatostatin [or somatotropin release-inhibiting factor (SRIF)] receptors, sst_2A, and studied the modulatory action of SRIF on voltage-gated K⁺ and Ca²⁺ currents in rod and cone photoreceptors. SRIF immunostaining was observed in widely spaced amacrine cells, whose perikarya are at the border of the inner nuclear layer and inner plexiform layer. sst_2A immunostaining was seen in the inner segments and terminals of rod and cone photoreceptors. Additional sst_2A immunoreactivity was expressed by presumed bipolar and amacrine cells. SRIF, at concentrations of 100–500 nm, enhanced a delayed outwardly rectifying K⁺ current (I_K) in both rod and cone photoreceptors. SRIF action was blocked in cells pretreated with pertussis toxin (PTX) and was substantially reduced by intracellular GDP_βS. Voltage-gated L-type Ca²⁺currents in rods and cones were differently modulated by SRIF. SRIF reduced Ca²⁺ current in rods by 33% but increased it in cones by 40%, on average. Both effects were mediated via G-protein activation and blocked by PTX. Ca²⁺-imaging experiments supported these results by showing that 500 nm SRIF reduced a K⁺-induced increase in intracellular Ca²⁺ in rod photoreceptor terminals but increased it in those of cones. Our results suggest that SRIF may play a role in the regulation of glutamate transmitter release from photoreceptors via modulation of voltage-gated K⁺ and Ca²⁺ currents.