A 4D HCCH-TOCSY experiment for assigning the side chain1H and13C resonances of proteins
- 1 November 1992
- journal article
- Published by Springer Nature in Journal of Biomolecular NMR
- Vol. 2 (6), 655-659
- https://doi.org/10.1007/bf02192854
Abstract
A 4D HCCH-TOCSY experiment is described for correlating and assigning the1H and13C resonances of protein amino acid side chains that has several advantages over 3D versions of the experiment. In many cases, both the1H and13C chemical shifts can be obtained in the 4D experiment from a simple inspection of the13C(ω3),1H(ω4) planes extracted at the1Hα(ω1)/13Cα(ω2) chemical shifts. Together with the 3D and 4D triple resonance experiments, this allows sequence-specific assignments to be obtained. In addition, the increased resolution of the 4D experiment compared to its 3D counterpart allows. automation of resonance assignments.Keywords
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