Multiple transmitter neurons inTritonia. III. Modulation of central pattern generator controlling feeding

Abstract

Feeding behavior in the gastropod mollusc Tritonia diomedea is controlled by a central pattern generator (CPG) in the buccal ganglia. The medially located, large dorsal white cells (B11) have been shown to contain two small cardioactive peptides (SCPs). A smaller nearby neuron (B12) also appears to contain the SCPs. B11's have also been shown to contain acetycholine (ACh), whereas B12's do not. We have shown earlier that intracellular stimulation of B11's drives contractions of the foregut. Here we show that intracellular electrical stimulation of B11's also elicits excitation of neurons B5 and stimulates the patterned motor output of the CPG. We showed earlier that B12's also stimulate contractions in the foregut, but they are in the opposite direction from those elicited by B11. We show here that electrical stimulation of B12's inhibits the output of the CPG. We showed earlier that superfusion of the isolated gut with SCP_B enhances peristalsis, and here we report that superfusion of the buccal ganglion with SCP_B elicits enhanced coordinated motor output from the CPG. The peptide has two effects on the bursting output of motor neurons. It produces an increase in (1) the rate of bursting and (2) the spike frequency during each burst. On the other hand, we reported earlier that ACh applied directly to isolated foregut inhibits ongoing peristalsis. Here we demonstrate that ACh superfusion of the buccal ganglion also inhibits the CPG output. Our evidence supports the view that in addition to stimulating foregut contractility, B11's modulate the output of the swallowing CPG by releasing a peptide from central terminals. We suggest roles for B11, B12, the SCPs, and ACh in controlling both central and peripheral aspects of feeding behavior.