Homogeneous Fluoroimmunoassay Using Lucifer Yellow VS: Determination of Albumin Plasma
- 1 January 1984
- journal article
- research article
- Published by SAGE Publications in Annals of Clinical Biochemistry: International Journal of Laboratory Medicine
- Vol. 21 (1), 59-63
- https://doi.org/10.1177/000456328402100111
Abstract
Non-separation fluoroimmunoassays are well suited to automation. The potential sensitivity of such assays has not been realised because the most commonly used label (fluorescein) has absorption and emission spectra which coincide with those of blood constituents and because it has a very small Stokes shift. Lucifer yellow VS has a larger Stokes shift than fluorescein and an emission maximum at longer wavelength. We describe an energy transfer fluoroimmunoassay for plasma albumin in which Lucifer yellow VS is used to label albumin and rhodamine B isothiocyanate is used to label anti-albumin antibodies. The assay shows good correlation with a dye-binding method for albumin and has sensitivity and precision which compare favourably with similar assays using a fluorescein label.This publication has 11 references indexed in Scilit:
- Use of Lucifer Yellow VS as a Label in Fluorescent Immunoassays Illustrated by the Determination of Albumin in SerumAnnals of Clinical Biochemistry: International Journal of Laboratory Medicine, 1983
- A Review of Fluoroimmunoassay and Immunofluorometric AssayAnnals of Clinical Biochemistry: International Journal of Laboratory Medicine, 1981
- Fluorescence quenching immunoassay of serum cortisolSteroids, 1980
- Automation of an energy-transfer immunoassay by using stopped-flow injection analysis with merging zonesAnalytica Chimica Acta, 1980
- Communication. Fluorescamine and fluorescein as labels in energy-transfer immunoassayThe Analyst, 1980
- Fluorescence protection immunoassay: a new homogeneous assay technique.Clinical Chemistry, 1979
- Non-separation fluoroimmunoassay of human albumin in biological fluidsClinica Chimica Acta; International Journal of Clinical Chemistry, 1978
- Enhancement fluoroimmunoassay of thyroxineFEBS Letters, 1977
- Fluorescence polarization immunoassay. Theory and experimental methodImmunochemistry, 1973
- Ultramicroestimation of human serum albumin: Binding of the cationic dye, 5,5′-dibromo-o-cresolsulfonphthaleinMicrochemical Journal, 1970