Plant small nuclear RNAs. V. U4 RNA is present in broad bean plants in the form of sequence variants and is base-paired with U6 RNA
Open Access
- 1 January 1988
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 16 (12), 5407-5426
- https://doi.org/10.1093/nar/16.12.5407
Abstract
U4 RNA, which is known to play an indispensable role in pre-mRNA splicing, is present in plant nuclei, has a canonical m32,2,7G cap at its 5′ end and is associated with U6 RNA in snRNP particles. It occurs in broad bean in the form of a number of sequence variants. Two of these were sequenced: U4A RNA is 154 and U4B RNA is 152 nucleotides long. Sequence similarity of broad bean U4B RNA is 94 per cent to broad bean U4A RNA, 65 per cent to rat U4A RNA, 61 per cent to Drosophila U4A RNA and 50 per cent to snR14, the U4 RNA equivalent of the yeast Saccharomyces cerevisiae. Sequence conservation is much more pronounced in the 5′ half of the molecule than in its 3′ half. The secondary structure of both variants of broad bean U4 RNA perfectly fits with that of all other 114 RNAs sequenced so far. Nucleotide changes between broad bean U4A and U4B RNAs are restricted to molecular regions that affect the thermodynamic stability of these molecules. A model is proposed for the base pairing interaction of broad bean U4 RNA with broad bean U6 RNA. This is the first report on the structure of a plant U4 RNA.This publication has 47 references indexed in Scilit:
- Analysis of RNase-A-resistant regions of adenovirus 2 major late precursor-mRNA in splicing extracts reveals an ordered interaction of nuclear components with the substrate RNAJournal of Molecular Biology, 1987
- High level of complexity of small nuclear RNAs in fungi and plantsJournal of Molecular Biology, 1987
- Interactions between small nuclear ribonucleoprotein particles in formation of spliceosomesCell, 1987
- The transcription of Xenopus laevis embryonic U1 snRNA genes changes when oocytes mature into eggs.Genes & Development, 1987
- Differential accumulation of U1 and U4 small nuclear RNAs during Xenopus development.Genes & Development, 1987
- Localization of a base-paired interaction between small nuclear RNAs U4 and U6 in intact U4/U6 ribonucleoprotein particles by psoralen cross-linkingJournal of Molecular Biology, 1985
- The small nuclear RNAs of DrosophilaJournal of Molecular Biology, 1984
- Molecular characterization of ribonucleoprotein antigens bound by antinuclear antibodies. A diagnostic evaluationArthritis & Rheumatism, 1982
- The use of thin acrylamide gels for DNA sequencingFEBS Letters, 1978
- Detection of specific sequences among DNA fragments separated by gel electrophoresisJournal of Molecular Biology, 1975