Phosphate transport in the proximal convolution of the rat kidney
- 1 January 1978
- journal article
- research article
- Published by Springer Nature in Pflügers Archiv - European Journal of Physiology
- Vol. 375 (1), 97-103
- https://doi.org/10.1007/bf00584153
Abstract
Proximal inorganic phosphate (P i ) transport was evaluated using the standing droplet method with simultaneous microperfusion of the peritubular blood capillaries. In chronic parathyroidectomized (PTX) rats addition of 3 μM of the Ca2+ ionophore A 23187 to the luminal perfusate had no effect on the P i transport, although the isotonic fluid reabsorption was reduced by 20%. When the Ca2+ concentration in the perfusates was raised from 1.5 mM to 3.0 mM the reabsorption did not change significantly. But when Ca2+ was omitted from the perfusates the P i reabsorption dropped by 19%, and when 2 mM EDTA were added to the perfusates P i transport decreased by 35%. The influx of P i from the interstitial space and from the cell into the phosphate-free luminal perfusate did not change, when the perfusates were Ca2+-free, but it increased by 23% in the presence of 2 mM EDTA. The data indicate that 1. a rise in intracellular Ca2+ above normal is not a factor which modifies “basal” P i transport i.e. when P i transport is independent of the action of parathyroid hormone. 2. A reduction of extracellular Ca2+ concentration from normal toward zero reduces P i transport without changing the paracellular leak permeability for P i . 3. With EDTA the paracellular leak permeability for P i is increased, thus causing an even greater reduction in net P i transport than with Ca2+-free solutions alone.Keywords
This publication has 18 references indexed in Scilit:
- Effect of divalent cation ionophore (A 23187) on renal handling of phosphorusKidney International, 1977
- Phosphate transport in the proximal convolution of the rat kidneyPflügers Archiv - European Journal of Physiology, 1977
- Coupling between proximal tubular transport processesPflügers Archiv - European Journal of Physiology, 1977
- Evidence for a parathyroid hormone-independent calcium modulation of phosphate transport along the nephron.JCI Insight, 1976
- Effects of ethylenediaminetetraacetate (EDTA) on urinary excretion of hydroxyproline, calcium and phosphorus in the ratCalcified Tissue International, 1974
- Ultraviolet and fluorescent spectral properties of the divalent cation ionophore A23187 and its metal ion complexesBiochemistry, 1974
- Direct Inhibitory Effect of Hypercalcemia on Renal Actions of Parathyroid HormoneJCI Insight, 1974
- Renal cortical adenyl cyclase: Effect of parathyroid hormone and calciumMetabolism, 1969
- Regulation of Parathyroid Hormone Secretion: Proportional Control by Calcium, Lack of Effect of Phosphate1Endocrinology, 1968
- Thyrocalcitonin, EGTA, and Urinary Electrolyte Excretion*JCI Insight, 1967