Potentiation of IgE Response in Vitro by T Cells from Rats Infected with Nippostrongylus Brasiliensis
Open Access
- 1 August 1979
- journal article
- research article
- Published by The American Association of Immunologists in The Journal of Immunology
- Vol. 123 (2), 918-924
- https://doi.org/10.4049/jimmunol.123.2.918
Abstract
The effect of T cells from rats infected with Nippostrongylus brasiliensis (Nb) on the IgE response was studied by using an in vitro system. Mesenteric lymph node cells from DNP-OA (dinitrophenyl derivatives of ovalbumin)-primed rats were cultured with DNP-OA or DNP-HSA in the presence of T or B cells from Nb-infected rats. It was found that T cells from the infected rat, but not normal T cells, selectively enhanced the IgE-forming cell response of DNP-OA primed cells to homologous antigen without affecting the IgG-forming cell response. The enhancement of the IgE response requires carrier-primed T cells; T cells from Nb-infected animals failed to enhance the Ig-forming cell response of DNP-OA primed cells to DNP-HSA. Culture supernatants of T cells from Nb-infected rats selectively enhanced the IgE response of DNP-OA primed cells to homologous antigen without affecting IgG response. The IgE-potentiating factor present in the unstimulated cultures is different from T cell-replacing factor(s), which was obtained by stimulation of antigen-primed T cells with antigen. When T cells from Nb-infected rats were incubated with Nb antigen, culture supernatant of the cells enhanced both IgE and IgG responses of DNP-OA primed cells to DNP-OA or DNP-HSA. It was also found that the molecular size of IgE-potentiating factor was significantly smaller than T cell-replacing factor. The results suggested that an optimal IgE response may require two subsets of T cells; one is carrier-specific T cells, which are common for all isotypes, and another subset is cells forming IgE-potentiating factor.This publication has 6 references indexed in Scilit:
- Idiotype‐specific T helper cells are required to induce idiotype‐positive B memory cells to secrete antibodyEuropean Journal of Immunology, 1978
- IGE-B CELL GENERATING FACTOR FROM LYMPH-NODE CELLS OF RATS INFECTED WITH NIPPOSTRONGYLUS-BRASILIENSIS .1. SOURCE OF IGE-B CELL GENERATING FACTOR1978
- IGE FORMATION IN RAT FOLLOWING INFECTION WITH NIPPOSTRONGYLUS-BRASILIENSIS .3. SOLUBLE FACTOR FOR GENERATION OF IGE-BEARING LYMPHOCYTES1977
- T-cell regulation of antibody responses: demonstration of allotype-specific helper T cells and their specific removal by suppressor T cells.The Journal of Experimental Medicine, 1976
- Rat T Lymphocyte-Specific Antigens and Their Cross-Reactivity with Mouse T CellsThe Journal of Immunology, 1976
- Is There Evidence for a Non-Antigen Specific Diffusable Chemical Mediator from the Thymus-Derived Cell in the Initiation of the Immune Response?**Supported by U. S. Public Health Service Research Grant AI-08795-03 and American Cancer Society Research Grant E-395-D.Published by Elsevier ,1971