INTERACTION OF OESTRADIOL AND NAFOXIDINE HYDROCHLORIDE WITH PROGESTERONE IN DECIDUOMATA FORMATION

Abstract

The effect of nafoxidine HC1 and estradiol on progesterone and estradiol uptake by rate deciduomata was studied. Rats were ovariectomized and their uteri traumatized; they were subsequently injected for 4 days with 1.5 mg of progesterone and either estradiol (0.005, 0.05, 0.1, 0.5 or 1.0 [mu]g), or es tradiol (0.05 [mu]g) and nafoxidine HC1 (0.5, 5, 50, 500, 1000 [mu]g). Tri-tiated estradiol or progesterone was injected 4 hr before killing the rats on the last day of treatment. Uterine horns (traumatized and con-tralateral non-traumatized cornua) were removed and immediately prepared for the determination of radio-activity. Total non-volatile radio-activity and the radio-activity associated with the protein, ether-soluble and water-soluble fractions were determined. Ether soluble fractions were chromatographed on silica gel; areas associated with major steroid components were isolated and counted for radio-activity. Both nafoxidine HC1 and estradiol effectively suppress the deciduomata response. The effects of each compound on the above parameters are not comparable in all cases. These differences, and their significance, are discussed.