Solubilization and Activity of Proteins in Compressible-Fluid Based Microemulsions
- 1 December 1992
- journal article
- Published by Springer Nature in Nature Biotechnology
- Vol. 10 (12), 1584-1588
- https://doi.org/10.1038/nbt1292-1584
Abstract
We have used the pressure dependency of density in near critical propane to design a novel protein extraction system. Active, structurally intact, proteins have been extracted from an aqueous phase into subcritical propane containing the non-ionic detergent polyoxyethylene sorbitan trioleate (Tween-85). The pressure dependence of protein transfer from the aqueous to organic phase is described for cytochrome C and subtilisin. The effect of the microemulsion and compressible propane on protein structure and function is also discussed. The first determinations of kinetic constants for an enzyme-catalyzed reaction in such a system are also presented.Keywords
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