Growth of Rabies Virus in Non-Nervous Tissue Culture

Abstract

Fixed rabies virus was propagated in hamster kidney tissue cultures through 15 serial passages. No cytopathic changes were observed in the infected cell cultures. Maximum virus titers in the tissue culture fluids were usually reached by the 10th to 15th day after inoculation. Virus could be recovered from cultures 91 days after inoculation. One of three attempts to propagate street rabies virus in hamster kidney cell cultures was successful. Street rabies virus produced no cytopathic changes in the cell cultures.