Heterogeneity of protein kinase C expression and regulation in T lymphocytes
- 15 January 1990
- journal article
- Published by Wiley in FEBS Letters
- Vol. 260 (1), 53-56
- https://doi.org/10.1016/0014-5793(90)80064-p
Abstract
The purpose of the present study was to examine protein kinase C (PKC) isotype expression in T lymphoblasts derived from peripheral blood and the T leukaemic cell Jurkat. Using antisera reactive with PKC α,β1, and β2 and γ, it was observed that T cells expressed two PKC isotypes, PKC α and β. No PKC γ was detected in T lymphocytes. In lymphoblasts, high levels of PKC β compared to PKC α were found whereas Jurkat cells expressed high levels of α compared to PKC β. Differences in the calcium sensitivity of phorbol ester‐induced phosphorylation were observed in Jurkat and T lymphoblasts which correlated with the relative levels of PKC α and β isotypes expressed by the cells.Keywords
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