Optical imaging of pancreatic beta cells in living mice expressing a mouse insulin I promoter‐firefly luciferase transgene
- 7 September 2005
- Vol. 43 (2), 80-86
- https://doi.org/10.1002/gene.20157
Abstract
We generated transgenic mice expressing firefly (Photinus pyralis) luciferase (luc) under the control of the mouse insulin I promoter (MIP). The mice have normal glucose tolerance and pancreatic islet architecture. The luciferase‐expressing beta cells can be readily visualized in living mice using whole‐body bioluminescent imaging. The MIP‐luc transgenic mice may be useful for monitoring changes in beta cell function or mass in living animals with normal or altered metabolic states. genesis 43:80–86, 2005.This publication has 18 references indexed in Scilit:
- Assessment of Pancreatic Islet Mass after Islet Transplantation Using In Vivo Bioluminescence ImagingTransplantation, 2005
- Cellular sources of new pancreatic β cells and therapeutic implications for regenerative medicineNature Cell Biology, 2004
- Kinetic analysis and modeling of firefly luciferase as a quantitative reporter gene in live mammalian cellsBiotechnology & Bioengineering, 2004
- Adult pancreatic β-cells are formed by self-duplication rather than stem-cell differentiationNature, 2004
- Proinsulin Expression by Hassall’s Corpuscles in the Mouse ThymusDiabetes, 2004
- Increased Islet Volume but Unchanged Islet Number in ob/ob MiceDiabetes, 2003
- Transgenic mice with green fluorescent protein-labeled pancreatic β-cellsAmerican Journal of Physiology-Endocrinology and Metabolism, 2003
- Insulin Expression Levels in the Thymus Modulate Insulin-Specific Autoreactive T-Cell ToleranceDiabetes, 2002
- Differential expression of the two nonallelic proinsulin genes in the developing mouse embryo.Proceedings of the National Academy of Sciences, 1993
- Method for the Isolation of Intact Islets of Langerhans from the Rat PancreasDiabetes, 1967