Purification and Some Properties of γ-Glutamyl Transpeptidase from Azo Dye-induced Hepatoma

Abstract

γ-Glutamyl transpeptidase [EC 2.3.2.2] was purified from azo dye-induced hepatoma of rats and its molecular and catalytic properties were studied. The purified enzyme appeared to be homogeneous as judged by ultracentrifugation and disc electrophoresis. The molecular weight was determined to be 113,000. The enzyme was a glycoprotein containing approximately 36% carbohydrate. The partial specific volume was 0.710 and the sedimentation coefficient was 5.60 S. The molecular activity of the enzyme was 20,500 at 37°C. The purified enzyme was very similar to hog and beef kidney enzymes in catalytic properties.