ELECTROPHORETIC CHARACTERIZATION OF HUMAN DEHYDROGENASES

Abstract

Several isoenzymes of lactate, malate, and isocitrate dehydrogenases have been demonstrated in human skeletal muscle, heart, and liver. Single zones of activity were shown for glucose-6-phosphate and glutamate dehydrogenases. A zone possessing a marked reducing capacity towards nicotinamide adenine dinucleotide and nicotinamide adenine dinucleotide phosphate was visualized in the absence of any substrate, using the tetrazolium-coupled histochemical method. This zone represents the so-called "nothing dehydrogenase".