Molecular sizes of photolabeled GABA and benzodiazepine receptor proteins are identical
- 24 January 1983
- journal article
- Published by Wiley in FEBS Letters
- Vol. 151 (2), 277-280
- https://doi.org/10.1016/0014-5793(83)80086-6
Abstract
[3H]Muscimol was irreversibly incorporated into rat cerebellar membranes upon irradiation with ultraviolet light. GABA agonists and antagonists inhibited this incorporation. The reversible muscimolbinding decreased after photoaffinity‐labeling of the membranes with muscimol. These results indicated that this irreversible incorporation is to the GABA receptor. Photolabeled GABA receptor protein showed its Mr of 50000±1000 in SDS—polyacrylamide gel electrophoresis. This molecular size is identical to that of the benzodiazepine receptor which was photolabeled with 3H‐flunitrazepam.Keywords
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