Colorectal cancer cell growth inhibition by linoleic acid is related to fatty acid composition changes
- 9 December 2010
- journal article
- Published by Zhejiang University Press in Journal of Zhejiang University-SCIENCE B
- Vol. 11 (12), 923-930
- https://doi.org/10.1631/jzus.b1000125
Abstract
Polyunsaturated fatty acids (PUFAs) possess anti-cancer action both in vitro and in vivo. In the present study, we detected cell viability with methyl thiazolyl tetrazolium (MTT) assay and cell membrane permeability with propidium iodide (PI) fluorescence dyeing, and calculated cell membrane fluidity change as fluorescence anisotropy. Fatty acid content in cells was measured by gas chromatography/mass spectroscopy (GC/MS), and the relationship between fatty acid composition and cell viability was studied. We observed that n-6 PUFA linoleic acid (LA) inhibited tumor cell growth at high concentrations (≥300 μmol/L), while low concentrations (100–200 μmol/L) seemed to promote cell proliferation. Analyses of cell membrane permeability, cell membrane fluidity, and cell fatty acid composition suggested that the anti-cancer action of LA could be related to changes in the ratio of n-6 to n-3 PUFAs. We observed that pre-incubation of cancer cells with 100 μmol/L LA for 24 h enhanced cell sensitivity to the cytotoxic action of LA, whereas undifferentiated cell line LoVo seemed to have a distinct path in LA-induced death. These results showed that one of the mechanisms by which supplementation of LA induces cancer cell death could be altering the ratio of n-6/n-3 PUFAs, and this may be related to cell differentiation status.Keywords
This publication has 26 references indexed in Scilit:
- Effect of free fatty acids and lysolipids on cellular uptake of doxorubicin in human breast cancer cell linesAnti-Cancer Drugs, 2010
- Effects of resorcylidene aminoguanidine (RAG) on selected parameters of isolated rat liver mitochondriaChemico-Biological Interactions, 2008
- Opposing Effects of n-6 and n-3 Polyunsaturated Fatty Acids on Pancreatic Cancer GrowthPancreas, 2008
- Mechanisms by which docosahexaenoic acid and related fatty acids reduce colon cancer risk and inflammatory disorders of the intestineChemistry and Physics of Lipids, 2008
- Modulation of prostate cancer genetic risk by omega-3 and omega-6 fatty acidsJCI Insight, 2007
- Differential Effects of Omega-3 and Omega-6 fatty Acids on Gene Expression in Breast Cancer CellsBreast Cancer Research and Treatment, 2006
- Essential fatty acids: biochemistry, physiology and pathologyBiotechnology Journal, 2006
- Prostate Tumor Growth and Recurrence Can Be Modulated by the ω-6:ω-3 Ratio in Diet: Athymic Mouse Xenograft Model Simulating Radical ProstatectomyNeoplasia, 2006
- Quantitative Determination of Plasma C8–C26 Total Fatty Acids for the Biochemical Diagnosis of Nutritional and Metabolic DisordersMolecular Genetics and Metabolism, 2001
- Cytotoxic effects of essential fatty acids (EFA) in mixed cultures of normal and malignant human cellsProgress in Lipid Research, 1986