SEPARATION AND CHARACTERIZATION OF TWO RAT-INTESTINAL AMYLASES

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Abstract
Rat small-intestinal mucosa contains two amylases, one an endoamylase ([alpha]-amylase) and one an exoamylase (glucamylase, [gamma]-amylase). The endoamylase accounts for the major part of the total amylase activity of the mucosa. It is chiefly present in a soluble form in mucosal homo-genates and has no maltase activity. The glucamylase is chiefly particle-bound in mucosal homogenates. It can be solubilized by papain-digestion of the particles. This enzyme hydrolyses maltose at approximately the same rate as starch. It accounts for about half of the total maltase activity of the mucosa. It has no isomaltase or invertase activity.