Phenotypic modulation of endothelial cells by transforming growth factor-beta depends upon the composition and organization of the extracellular matrix.
Open Access
- 31 March 1988
- journal article
- research article
- Published by Rockefeller University Press in The Journal of cell biology
- Vol. 106 (4), 1375-1384
- https://doi.org/10.1083/jcb.106.4.1375
Abstract
Transforming growth factor beta (TGF-.beta.) is angiogenic in vivo. In vitro, endothelial cell proliferation is inhibited by TGF-.beta.. We have correlated this inhibitory effect with an increase in cellular fibronectin synthesis and deposition in a two-dimensional culture system using specific matrix coatings. The inhibitory effect was mimicked by addition of soluble fibronectin to cultures. In contrast, TGF-.beta. was found to elicit the formation of tube-like structures (mimicking angiogenesis) when microvascular endothelial cells were grown in three-dimensional collagen gels. In this culture system TGF-.beta. elicited rapid extensive formation of complex, branching, tube-like structures, while cell proliferation was not inhibited. These data confirm and support the hypothesis that TGF-.beta. is angiogenic and may exert some of its effects through modulation of matrix synthesis and are consisted with the hypothesis that the organization of the extracellular environment influences cellular responses to this "panregulin.".This publication has 38 references indexed in Scilit:
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