Partial Isolation and Characterization off Antigen(s) Associated With Murine Melanoma 2

Abstract

Antigen(s) associated with murine B16 melanoma (MAA) were radiolabeled by the incubation of tumor cells with ³H-leucine and identified by an antigen-binding radioimmunoassay. The MAA had a molecular weight of 150,000–200,000 daltons, were precipitable by 52% ammonium sulfate, and were inactivated by treatment with heat, acid, base, trypsin, or papain. Treatment with neuraminidase greatly increased MAA antigenicity. These results suggest that MAA are glycoproteins. Some MAA were present to a lesser and variable extent in normal syngeneic murine fibroblasts. MAA were released into culture medium by tumor cells and to a lesser extent by normal cells.