Role of Na+ and Li+ in thiomethylgalactoside transport by the melibiose transport system of Escherichia coli
- 1 April 1978
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 134 (1), 147-156
- https://doi.org/10.1128/jb.134.1.147-156.1978
Abstract
Thiomethyl-.beta.-galactoside (TMG) accumulation via the melibiose transport system was studied in lactose transport-negative strains of E. coli. TMG uptake by intact cells or membrane vesicles was markedly stimulated by Na+ or Li+ between pH 5.5-8. The Km for uptake of TMG was approximately 0.2 mM at an external Na+ concentration of 5 mM (pH 7). The .alpha.-galactosides, melibiose, methyl-.alpha.-galactoside and o-nitrophenyl-.alpha.-galactoside had a high affinity for this system but lactose, maltose and glucose had none. Evidence is presented for Li+-TMG or Na+-TMG cotransport.This publication has 47 references indexed in Scilit:
- Co-transport of Na+ and methyl-β-D-thiogalactopyranoside mediated by the melibiose transport system of Escherichia coliBiochemical and Biophysical Research Communications, 1977
- Na+‐dependent uptake of amino acids by an alkalophilic bacillusFEBS Letters, 1976
- Sodium‐dependent glutamate transport in membrane vesicles of Escherichia coli K‐12FEBS Letters, 1975
- Na+-dependent amino acid transport in isolated membrane vesicles of a marine pseudomonad energized by electron donorsBiochemical and Biophysical Research Communications, 1972
- A sodium-dependent sugar co-transport system in bacteriaBiochemical and Biophysical Research Communications, 1971
- Transport systems for galactose and galactosides in Escherichia coliJournal of Molecular Biology, 1968
- α-Galactosidase activity in cell-free extracts of Escherichia coliBiochemical and Biophysical Research Communications, 1966
- A second permease for methyl-thio-β-d-galactoside in Escherichia coliBiochimica et Biophysica Acta (BBA) - General Subjects, 1965
- Optimal conditions for mutagenesis by N-methyl-N′-nitro-N-nitrosoguanidine in escherichia coli K12Biochemical and Biophysical Research Communications, 1965
- Na+ dependent active transport of α-aminoisobutyric acid into cells of a marine pseudomonadBiochemical and Biophysical Research Communications, 1963