Further Properties of Adenosine Triphosphatase and β-Glycerophosphatase from Barley Roots

Abstract

Cell wall preparations from barley roots contain ATP-ase activity that is stimulated by monovalent cations at alkaline pH values, above that obtained with calcium or magnesium ions. Sodium is the most effective cation followed by potassium, lithium, and rubidium. Similar activation is obtained with a soluble enzyme fraction and with excised root tips. β-Glycerophosphatase shows no stimulation by calcium and sodium or potassium have only a small stimulatory effect. Disc electrophoresis demonstrates the group character of ATP-ase and β-glycerophosphatase activities which consist of multiple forms either specific to one or other substrate or hydrolysing both.