T4 polynucleotide ligase catalyzed joining on triple-stranded nucleic acids

Abstract

DT10 will form triple-stranded complexes with dAn and these complexes can serve as substrate for [phage] T4 polynucleotide ligase (EC 6.5.1.1). The rate of phosphodiester formation was approximately the same as for the double-stranded complex and the rate appears to be similar on the 2 strands in the complex. Joining of dT10 also took place in the presence of the double-stranded complexes dAn.cntdot.dTn and dAn.cntdot.rUn. Polyamines increase the rate of joining catalyzed by T4 polynucleotide ligase under certain conditions.