Evidence for a similar compartmentation of recaptured and endogenously synthesized dopamine in striatal synaptosomes
- 1 February 1980
- journal article
- research article
- Published by Springer Nature in Neurochemical Research
- Vol. 5 (2), 115-121
- https://doi.org/10.1007/bf00964326
Abstract
The aim of the present study was to compare the release pattern of [3H]dopamine ([3H]DA) originated from [3H]tyrosine or by uptake in striatal synaptosomes. Synaptosomes prelabeled either with [3H]DA or with [3H]tyrosine were superfused in three conditions stimulating DA release by different mechanisms: (1) depolarization with high K+; (2) inversion of the Na+ gradient across the plasma membrane; (3) exposure tod-amphetamine. Since DA contained in different pools may exit from nerve endings by different processes, DA release was analyzed in the presence or in the absence of nomifensine which allows discrimination between carrier-mediated and carrier-independent processes. The pattern of DA release in the three conditions tested was identical, whether [3H]DA originated from synthesis or from uptake. Nomifensine did not affect the high-K+-induced release and inhibited that induced by the other two stimuli. The results suggest that newly synthesized and recaptured DA have a similar compartmentation in nerve endings.Keywords
This publication has 12 references indexed in Scilit:
- Release of endogenous catecholamines from isolated rat brain tissue by fenfluramine and N-ethylamphetamine—effects of pargylineBiochemical Pharmacology, 1978
- Effect of veratrine alkaloids on the efflux of extragranular noradrenaline from rabbit atriaJournal of Neurochemistry, 1976
- A STUDY OF THE METABOLISM AND RELEASE OF DOPAMINE AND AMINO ACIDS FROM NERVE ENDINGS ISOLATED FROM SHEEP CORPUS STRIATUMJournal of Neurochemistry, 1976
- A method for concurrent measurement of picomole quantities of acetylcholine, choline, dopamine, norepinephrine, serotonin, 5-hydroxytryptophan, 5-hydroxyindoleacetic acid, tryptophan, tyrosine, glycine, aspartate, glutamate, alanine, and gamma-aminobutyric acid in single tissue samples from different areas of rat central nervous systemAnalytical Biochemistry, 1975
- Nomifensine: a new potent inhibitor of dopamine uptake into synaptosomes from rat brain corpus striatumJournal of Pharmacy and Pharmacology, 1974
- A simple apparatus for studying the release of neurotransmitters from synaptosomesEuropean Journal of Pharmacology, 1974
- GABA and glutamate uptake by subcellular fractions enriched in synaptosomes: Critical evaluation of some methodological aspectsBrain Research, 1973
- Dopamine: Spontaneous and drug-induced release from the caudate nucleus in the catBrain Research, 1971
- RELEASE OF NEWLY SYNTHESIZED DOPAMINE FROM DOPAMINE-CONTAINING TERMINALS IN THE STRIATUM OF THE RATProceedings of the National Academy of Sciences, 1969
- Selective release of newly synthesized norepinephrine from the cat spleen during sympathetic nerve stimulation.1968