Efficient gene activation in mammalian cells by using recombinant adenovirus expressing site-specific Cre recombinase
Open Access
- 11 October 1995
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 23 (19), 3816-3821
- https://doi.org/10.1093/nar/23.19.3816
Abstract
A recombinant adenovirus (Ad) expressing Cre recombinase derived from bacteriophage P1 was constructed. To assay the Cre activity in mammalian colls, another recombinant Ad bearing an on/off-switching reporter unit, where a LacZ -expression unit can be activated by the Cre-mediated exclsionai deletion of an interposed stuffer DNA, was also constructed. Co-infection experiments together with the Cre-expressing and the reporter recombinant Ads showed that the Cre-mediated switching of gene expression was detected in nearly 100% of cultured CV1, HeLa and Jurkat cells. These results suggest that the recombinant Ad efficiently expressed functional Cre and offers a basis for estabilshing a powerful on/off switching strategy of gene expression incultured mammalian cells and presumably in transgenic animals. The method is also applicable to construction of recombinant Ad bearing a gene the expression of which is deleterious to propagation of recombinant Ad.Keywords
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