The Assay of Acetylcholine on the Superfused Frog Rectus Muscle
- 1 September 1957
- journal article
- Published by Oxford University Press (OUP) in Journal of Pharmacy and Pharmacology
- Vol. 9 (1), 536-540
- https://doi.org/10.1111/j.2042-7158.1957.tb12307.x
Abstract
A method of superfusion for assaying acetylcholine on the eserinised frog rectus muscle applying each dose in a volume of 0·2 ml. is described. The sensitivity of the superfused preparation is compared with that of the preparation in a 5 ml. bath and is 10 to 20 times more sensitive than the bath preparation. Results of 10 parallel assays (2 + 2) suggest that the method of superfusion gives a reliable and accurate assay of acetylcholine.Keywords
This publication has 2 references indexed in Scilit:
- A method of conducting a biological assay on a preparation giving repeated graded responses illustrated by the estimation of histamineThe Journal of Physiology, 1942
- Observations on the relation of histamine to reactive hyperaemiaThe Journal of Physiology, 1941