Comparison of Anastomosis Groups ofRhizoctonia solaniby Polyacrylamide Gel Electrophoresis of Soluble Proteins

Abstract

Soluble proteins were extracted from isolates of the 5 anastomosis groups (AG) of R. solani and compared by electrophoresis in polyacrylamide gels. The protein profiles for the 5 AG were distinctive and could be distinguished from one another. Particular emphasis was placed on determining the variability of protein patterns of isolates belonging to AG3 and AG4 from different hosts [Gossypium hirsutum and Solanum tuberosum]and geographic locations. The protein patterns from isolates belonging to AG3 were uniformly distinct, regardless of isolate source. Protein patterns of isolates belonging to AG4 varied in minor bands, but the general pattern was recognizable as AG4. Polyacylamide gel electrophoresis of soluble proteins can be used to help distinguish anastomosis groups of R. solani.