The Similarity between the Primary Structures of Two Non‐histone Chromosomal Proteins
- 1 March 1976
- journal article
- Published by Wiley in European Journal of Biochemistry
- Vol. 62 (3), 461-469
- https://doi.org/10.1111/j.1432-1033.1976.tb10179.x
Abstract
A comparison has been made of two non-histone chromosomal proteins, high-mobility-group proteins 1 and 2. Peptides produced from the two proteins by various chemical and enzymic cleavages have been compared by polyacrylamide gel electrophoresis and peptide mapping. In addition the cysteine content, C-terminal sequences and amide content have been determined for both proteins. The results obtained, together with previous results, show that high-mobility-group proteins 1 and 2 have closely related primary structures, although obviously being different molecules. When analysed by isoelectric focusing both proteins exhibit complex banding patterns. In the case of high-mobility-group protein 1, this could be caused by aggregation, but with high-mobility-group protein 2 there are four distinctive subfractions focusing between pH 7.8 and 8.9.Keywords
This publication has 17 references indexed in Scilit:
- An improved large scale fractionation of high mobility group non-histone chromatin proteinsBiochimica et Biophysica Acta (BBA) - Protein Structure, 1975
- Isoelectric focusing in gelsJournal of Chromatography A, 1974
- In vivo occurence of bound ADP‐riboseFEBS Letters, 1973
- Isolation and Characterisation of Two Calf‐Thymus Chromatin Non‐Histone Proteins with High Contents of Acidic and Basic Amino AcidsEuropean Journal of Biochemistry, 1973
- Regeneration of methionyl residues from their sulfonium salts in peptides and proteinsBiochemistry, 1972
- Oxidation of the cysteine-containing histone F3. Detection of an evolutionary mutation in a conservative histoneBiochemistry, 1971
- The utilisation of i-dimethylaminonaphthalene-5-sulphonyl chloride for quantitative determination of free amino acids and partial analysis of primary structure of proteinsJournal of Chromatography A, 1970
- The C-terminal amino acids and sequences of calf and rat thymus histonesBiochimica et Biophysica Acta (BBA) - Protein Structure, 1969
- High resolution acrylamide gel electrophoresis of histonesArchives of Biochemistry and Biophysics, 1969
- Determination of C-Terminal Amino Acids and Peptides by Hydrazinolysis1Journal of the American Chemical Society, 1955