Suppression of Virus Accumulation in Transgenic Plants Exhibiting Silencing of Nuclear Genes.
Open Access
- 1 February 1996
- journal article
- Published by Oxford University Press (OUP) in Plant Cell
- Vol. 8 (2), 179-188
- https://doi.org/10.1105/tpc.8.2.179
Abstract
Homology-dependent gene silencing contributes to variation between transgenic plants with respect to transgene and/or endogenous gene expression levels. Recent studies have linked post-transcriptional gene silencing and virus resistance in plants expressing virus-derived transgenes. Using a potato virus X vector, we present three examples in which silencing of nonviral transgenes prevented virus accumulation. This effect was dependent on sequence homology between the virus and the silenced transgene. Analysis of potato virus X derivatives carrying bacterial [beta]-glucuronidase (GUS) sequences showed that the 3[prime] region of the GUS coding sequence was a target of the silencing mechanism in two independent tobacco lines. Methylation of the silenced GUS transgenes in these lines was also concentrated in the 3[prime] region of the GUS coding sequence. Based on this concurrence, we propose a link between the DNA-based transgene methylation and the RNA-based gene silencing process.Keywords
This publication has 19 references indexed in Scilit:
- Post-transcriptional cosuppression of beta-1,3-glucanase genes does not affect accumulation of transgene nuclear mRNA.Plant Cell, 1995
- Transgenic plant virus resistance mediated by untranslatable sense RNAs: expression, regulation, and fate of nonessential RNAs.Plant Cell, 1994
- Green Fluorescent Protein as a Marker for Gene ExpressionScience, 1994
- Induction of a Highly Specific Antiviral State in Transgenic Plants: Implications for Regulation of Gene Expression and Virus Resistance.Plant Cell, 1993
- Differences in DNA‐methylation are associated with a paramutation phenomenon in transgenic petuniaThe Plant Journal, 1993
- An Arabidopsis mutant with a reduced level of cab140 RNA is a result of cosuppression.Plant Cell, 1993
- Potato virus X as a vector for gene expression in plantsThe Plant Journal, 1992
- Flavonoid genes in petunia: addition of a limited number of gene copies may lead to a suppression of gene expression.Plant Cell, 1990
- Introduction of a Chimeric Chalcone Synthase Gene into Petunia Results in Reversible Co-Suppression of Homologous Genes in trans.Plant Cell, 1990
- Altered gene expression in plants due totrans interactions between homologous genesTrends in Biotechnology, 1990