Macromolecule Synthesis and Breakdown in Relation to Sporulation and Meiosis in Yeast
- 1 August 1974
- journal article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 119 (2), 619-28
- https://doi.org/10.1128/jb.119.2.619-628.1974
Abstract
The time course of synthesis and breakdown of various macromolecules has been compared for sporulating (a/alpha) and nonsporulating (a/a and alpha/alpha) yeast cells transferred to potassium acetate sporulation medium. Both types of cells incorporate label into ribonucleic acid and protein. The gel electrophoresis patterns of proteins synthesized in sporulation medium are identical for sporulating and nonsporulating diploids; both are different from electropherograms of vegetative cells. Sporulating and nonsporulating strains differ with respect to deoxyribonucleic acid synthesis; no deoxyribonucleic acid is synthesized in the latter case, whereas the deoxyribonucleic acid complement is doubled in the former. Glycogen breakdown occurs only in sporulating strains. Breakdown of preexisting vegetative ribonucleic acid and protein molecules occurs much more extensively in sporulating than in nonsporulating cells. A timetable of these data is presented.Keywords
This publication has 41 references indexed in Scilit:
- Physical mapping of the early region of bacteriophage T7 DNAJournal of Molecular Biology, 1973
- Analysis of bacteriophage T7 early RNAs and proteins on slab gelsJournal of Molecular Biology, 1973
- The genetic control of sporulation inSaccharomycesMolecular Genetics and Genomics, 1972
- Ammonium Repression of Extracellular Protease in Aspergillus nidulansJournal of General Microbiology, 1972
- RECOMBINATION IN YEASTAnnual Review of Genetics, 1971
- SPINDLES, SPINDLE PLAQUES, AND MEIOSIS IN THE YEAST SACCHAROMYCES CEREVISIAE (HANSEN)The Journal of cell biology, 1971
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970
- The ribosome cycle, nucleoli, and cytoplasmic nucleoloids in the meiocytes ofLiliumProtoplasma, 1970
- DNA Synthesis during Yeast Sporulation: Genetic Control of an Early Developmental EventScience, 1970
- THE METABOLISM OF YEAST SPORULATION: IV. CYTOLOGICAL AND PHYSIOLOGICAL CHANGES IN SPORULATING CELLSCanadian Journal of Microbiology, 1962