DNA-relaxing activity and endonuclease activity in Xenopus laevis oocytes.
- 1 December 1976
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 73 (12), 4551-4554
- https://doi.org/10.1073/pnas.73.12.4551
Abstract
Complete simian virus 40 DNA produced by a soluble cell-free extract derived from stage 6 oocytes of X. laevis consists of fully relaxed circles (i.e., with no superhelical turns). An endonuclease and a DNA-relaxing protein, either or both of which could be responsible for the relaxation of the complex DNA, were purified from the extract. The endonuclease(s) produces nicked circles (having a single-strand scission) and linear full-size molecules. The DNA-relaxing protein is in the nucleus, has a MW of approximately 70,000, and is able to remove both negative and positive superhelical turns.Keywords
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