Phagocytosis of murine lymphoma cells by macrophages. I. Factors affecting in vitro phagocytosis.
- 1 January 1971
- journal article
- Vol. 20 (1), 67-74
Abstract
Factors affecting in vitro phagocytosis of a DBA/2 lymphoma, L5178Y, were investigated. Peritoneal macrophages from mice injected with thioglycollate medium were found to take up more lymphoma cells than those from normal mice. The number of cells phagocytosed by normal macrophages was found to be related to the concentration of antiserum employed and the number of lymphoma cells added to each macrophage slip culture. Treatment of macrophages or lymphoma cells with either trypsin or neuraminidase prior to the opsonic test greatly increased the number of cells ingested and also the number of macrophages containing ingested lymphoma cells. The relevance of the results from this opsonic test system to in vivo findings is discussed.This publication has 6 references indexed in Scilit:
- Role of cellular and humoral factors in the destruction of nascent plasma cell tumors.1969
- The effect of neuraminidase on the immunogenicity of the Landschütz ascites tumour: site and mode of actionBritish Journal of Cancer, 1968
- The uptake of particulate antigens.1968
- Opsonization of Cells by Isoantibody in vitroNature, 1963
- POSSIBLE RELATIONSHIPS BETWEEN THE CYTOTOXIC EFFECTS OF ISOANTIBODY AND HOST CELL FUNCTION *Annals of the New York Academy of Sciences, 1960
- STUDIES OF THE CULTURE OF LEUKEMIC CELLS IN VITRO*Annals of the New York Academy of Sciences, 1958