Forward transport of glycoproteins on leading lamellipodia in locomoting cells
- 1 July 1989
- journal article
- Published by Springer Nature in Nature
- Vol. 340 (6231), 315-317
- https://doi.org/10.1038/340315a0
Abstract
In several types of locomoting cells, active rearward transport of particles on the cell surface has been observed and correlated with motility. No forward transport of particles has previously been reported, however. Here we report rapid forward transport of concanavalin A-coated gold particles on the dorsal surfaces of lamellipodia of fish epidermal keratocytes. These movements are active, not diffusive, and more rapid than either rearward particle transport or the rate of cell locomotion. We observed forward transport in migrating, but not in stationary cells, and could block the movement by treatment with cytochalasin D. These studies demonstrate for the first time that small numbers of glycoproteins can be actively transported on the surface of the cell to the front of the lamellipodium. We suggest that this mechanism transports proteins involved in cell locomotion, such as proteins necessary for adhesion, and could also produce an extensile force.Keywords
This publication has 21 references indexed in Scilit:
- Actions of cytochalasins on the organization of actin filaments and microtubules in a neuronal growth cone.The Journal of cell biology, 1988
- Tracking kinesin-driven movements with nanometre-scale precisionNature, 1988
- Centripetal transport of cytoplasm, actin, and the cell surface in lamellipodia of fibroblastsCell Motility, 1988
- Exchange of actin subunits at the leading edge of living fibroblasts: possible role of treadmilling.The Journal of cell biology, 1985
- Endocytosis: Relation to Capping and Cell LocomotionScience, 1984
- Behaviour and structure of the leading lamella in moving fibroblasts: I. Occurrence and centripetal movement of arcshaped microfilament bundles beneath the dorsal cell surfaceJournal of Cell Science, 1983
- Motion of particles adhering to the leading lamella of crawling cells.The Journal of cell biology, 1981
- Membrane and Cytoplasmic Changes in B Lymphocytes Induced by Ligand-Surface Immunoglobulin InteractionAdvances in Immunology, 1976
- The locomotion of fibroblasts in culture: III. Movements of particles on the dorsal surface of the leading lamellaExperimental Cell Research, 1970
- Surface Movements during the Growth of Single Explanted NeuronsProceedings of the National Academy of Sciences, 1970