Purification and characterization of human platelet von Willebrand factor
- 1 November 1994
- journal article
- Published by Wiley in British Journal of Haematology
- Vol. 88 (3), 582-591
- https://doi.org/10.1111/j.1365-2141.1994.tb05077.x
Abstract
Platelet von Willebrand factor (vWf) was purified from human platelet concentrates. The multimeric structure of the purified platelet vWf was similar to that observed in the initial platelet lysate, and, like the platelet lysate, the purified platelet vWf contained higher molecular weight multimers than plasma vWf. The apparent molecular weight of the reduced platelet vWf subunit was similar to the plasma vWf subunit. The N-terminal amino acid of the purified platelet and plasma vWf was blocked. In concentration dependent binding to botrocetin- or ristocetin-stimulated platelets, 125I-plasma vWf bound with a higher affinity than platelet. The ristocetin cofactor activity per mg of purified plasma vWf was 5-fold greater than the platelet vWf activity. Platelet and plasma vWf bound to collagen with similar affinities; however, platelet vWf bound to thrombin-stimulated platelets and to heparin with a higher affinity than plasma vWf. The differences in the binding affinity(s) of plasma and platelet vWf to platelet GPIb and GPIIb/IIIa and extracellular matrix proteins may reflect different roles for plasma and platelet vWf in the initial stages of haemostasis and thrombosis.Keywords
This publication has 37 references indexed in Scilit:
- O-linked carbohydrate of recombinant von Willebrand factor influences ristocetin-induced binding to platelet glycoprotein 1b.JCI Insight, 1992
- Effect of deletion of the A1 domain of von Willebrand factor on its binding to heparin, collagen and platelets in the presence of ristocetinEuropean Journal of Biochemistry, 1991
- Binding of von Willebrand factor to glycoproteins Ib and IIb/IIIa complex: affinity is related to multimeric sizeBritish Journal of Haematology, 1989
- Biosynthesis of von Willebrand protein by human megakaryocytes.JCI Insight, 1985
- Platelets have more than one binding site for von Willebrand factor.JCI Insight, 1983
- Active release of human platelet factor VIII-related antigen by adenosine diphosphate, collagen, and thrombin.JCI Insight, 1978
- Synthesis of Factor VIII Antigen by Cultured Guinea Pig MegakaryocytesJCI Insight, 1977
- Stabilization of Factor VIII in Plasma by the von Willebrand FactorJCI Insight, 1977
- Platelet membrane glycoproteins implicated in ristocetin-induced aggregation. Studies of the proteins on platelets from patients with Bernard-Soulier syndrome and von Willebrand's disease.JCI Insight, 1976
- Subcellular platelet factor VIII antigen and von Willebrand factor.The Journal of Experimental Medicine, 1975