Ingress of Salmonella enterica Typhimurium into Tomato Leaves through Hydathodes
Open Access
- 8 January 2013
- journal article
- biology
- Published by Public Library of Science (PLoS) in PLOS ONE
- Vol. 8 (1), e53470
- https://doi.org/10.1371/journal.pone.0053470
Abstract
Internal contamination of Salmonella in plants is attracting increasing attention for food safety reasons. In this study, three different tomato cultivars “Florida Lanai”, “Crown Jewel”, “Ailsa Craig” and the transgenic line Sp5 of “Ailsa Craig” were inoculated with 1 µl GFP-labeled Salmonella Typhimurium through guttation droplets at concentrations of 109 or 107 CFU/ml. Survival of Salmonella on/in tomato leaves was detected by both direct plating and enrichment methods. Salmonella cells survived best on/in the inoculated leaves of cultivar “Ailsa Craig” and decreased fastest on/in “Florida Lanai” leaves. Increased guttation in the abscisic acid over-expressing Sp5 plants may have facilitated the entrance of Salmonella into leaves and the colonization on the surface of tomato leaves. Internalization of Salmonella Typhimurium in tomato leaves through guttation drop inoculation was confirmed by confocal laser microscopy. For the first time, convincing evidence is presented that S. enterica can enter tomato leaves through hydathodes and move into the vascular system, which may result in the internal translocation of the bacteria inside plants.Keywords
This publication has 26 references indexed in Scilit:
- Internal Colonization of Salmonella enterica Serovar Typhimurium in Tomato PlantsPLOS ONE, 2011
- Colonization of Tomato Plants by Salmonella enterica Is Cultivar Dependent, and Type 1 Trichomes Are Preferred Colonization SitesApplied and Environmental Microbiology, 2011
- Percolation and Survival of Escherichia coli O157:H7 and Salmonella enterica Serovar Typhimurium in Soil Amended with Contaminated Dairy Manure or SlurryApplied and Environmental Microbiology, 2009
- Differential Attachment to and Subsequent Contamination of Agricultural Crops by Salmonella entericaApplied and Environmental Microbiology, 2008
- Role of Soil, Crop Debris, and a Plant Pathogen in Salmonella enterica Contamination of Tomato PlantsPLOS ONE, 2008
- Differential interaction of Salmonella enterica serovars with lettuce cultivars and plant-microbe factors influencing the colonization efficiencyThe ISME Journal, 2007
- Physiological and Molecular Responses of Lactuca sativa to Colonization by Salmonella enterica Serovar DublinApplied and Environmental Microbiology, 2007
- Recurrent multistate outbreak of Salmonella Newport associated with tomatoes from contaminated fields, 2005Epidemiology and Infection, 2007
- Outbreak ofSalmonellaBraenderup infections associated with Roma tomatoes, northeastern United States, 2004: a useful method for subtyping exposures in field investigationsEpidemiology and Infection, 2007
- Ingress of Clavibacter michiganensis subsp. michiganensis into Tomato Leaves Through HydathodesPhytopathology®, 1998