Application of HPLC to counting of colored microspheres in determination of regional blood flow
- 1 March 1998
- journal article
- research article
- Published by American Physiological Society in American Journal of Physiology-Heart and Circulatory Physiology
- Vol. 274 (3), H1041-H1047
- https://doi.org/10.1152/ajpheart.1998.274.3.h1041
Abstract
Colored microspheres have become popular compared with radioactive microspheres because they do not use radioactivity. However, they suffer from a much greater variability in their determination. We have developed a new method for assaying the dye using high-performance liquid chromatography (HPLC) with internal standard. This technique permits accurate determination of ≤400 spheres in rat blood, heart, kidney, liver, and brain with a relative error [coefficient of variation (CV)]Keywords
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