Liver and meat extracts were fractionated by the method of Cohn et al. so as to obtain fraction G. This fraction, effective in pernicious anemia, proved to contain most of the secretagogues found in whole liver and meat extract as well as much vasodilatin. The vasodilatin was next completely removed with Lloyd''s reagent. The resulting vasodilatin-free extracts, together with similar extracts made from the blood and lymph of fed and unfed dogs, were administered to 3 kinds of experimental animals: Dogs with (1) Pavlov pouch, (2) gastrostomy, Thiry fistula, and intact vagi, and (3) pouch of the entire stomach. Administration was by direct application to the gastric mucosa, by duodenal tube to the intestine, by introduction through the Thiry fistula, and by intravenous and intraperitoneal injection. The results showed that secretagogues applied to the gastric mucosa can act without passing into the blood-stream. In the intestine they act by being absorbed. Given intravenously they act, provided the dose needed for local gastric application is quadrupled. Intraperitoneal injection is also effective, but requires still larger doses. Atropine abolishes the responses to both local and intravenous administration. Beta-hydroxyproline, given locally or intravenously, does not excite the gastric glands.