HLA-DR TYPING BY RADIOIMMUNOASSAY

Abstract

A radioimmunoassay procedure was described by which peripheral blood lymphocytes can be typed for HLA-DR specificities. The major advantages of this method are the following: simple and reproducible procedure, no need for B [bone marrow-derived] lymphocyte separation, no need for optimal viability and no need for preabsorption of antisera with platelets. This method will find an application in the genetic and biochemical analysis of the HLA complex and in clinical tests of Ia [immune response-associated] antigens for diagnostic or prognostic purposes and in retrospective transplant studies.