Soluble biospecific macromolecule for purification of estrogen receptor.
- 1 July 1978
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 75 (7), 3143-3147
- https://doi.org/10.1073/pnas.75.7.3143
Abstract
An alternative to affinity chromotography purification of proteins based on the use of a water-soluble biospecific polymer was applied to the estrogen receptor from calf uterus. The receptor (4S-trypsin form) was bound to a dextran-estradiol conjugate (MW .apprx. 500,000) and the complex was isolated by gel filtration. Highly purified receptor was subsequently released from the dextran-estradiol conjugate by exchange with [3H]estradiol.This publication has 11 references indexed in Scilit:
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