A Naphthoquinone Adsorbent for Affinity Chromatography of Human Dihydropteridine Reductase

Abstract
A 1,2-naphthoquinone adsorbent is described which allows simple purification of [human and Macaca irus liver] dihydropteridine reductase [EC 1.6.99.7] directly from crude extract. The native MW indicates that a tetramer structure of the species is isolated by this method; this is unusual and possibly reflects the capacity of the procedure to preserve the native state of the enzyme.