Separation of Proteins by Thin-Layer Gel Filtration.

Abstract

A method for the separation of proteins by means of gel filtration applied as a thin layer chromatographic technique is described. Sephadex G-50 and G-75 are useful for the separation of relatively low molecular weight proteins whereas G-100 and G-200 permit separations of proteins with higher molecular weights, i.e. blood serum proteins. The method allows easy detection of enzymic activities after gel filtration.