Blockade of Steroid-Induced Luteinizing Hormone Release by Selective Depletion of Anterior Hypothalamic Norepinephrine Activity*

Abstract

Female Sprague-Dawley rats, weighing 200–225 g, were ovariectomized and, 10 days later, were given a single sc injection of 10 μg estradiol benzoate/100 g BW. Three days after estradiol benzoate treatment, animals received 50 μg progesterone (P)/100 g BW, resulting in a surge in LH release 7 and 9 h later. To determine the locus of the noradrenergic component of the P-induced LH surge, 6-hydroxydopamine (6-OH-DA), a neurotoxin, was implanted into either the suprachiasmatic region or the median eminence (ME) 24 h before P administration. An implant of 6-OH-DA in the suprachiasmatic region decreased anterior hypothalamic norepinephrine concentration by 83%, anterior hypothalamic dopamine concentration by 24%, and eliminated the P-induced LH surge. ME implant of 6-OH-DA decreased norepinephrine concentration by 57% without affecting dopamine concentration, but was unable to alter the Pinduced LH surge. These results indicate that the anterior hypothalamic noradrenergic system is necessary for the P-induced LH surge in ovariectomized rats and that noradrenergic nerve terminals in the ME are not involved in this process.